Kisspeptin-10

Kisspeptin-10 is a synthetic decapeptide representing the C-terminal active fragment of kisspeptin — the protein product of the KISS1 gene — and contains the essential RF-amide (Arg-Phe-NH2) C-terminal motif required for high-affinity GPR54 receptor binding. It is the minimal active kisspeptin fragment retaining full GPR54 agonist potency and is the most widely used kisspeptin research tool in published neuroendocrinology and reproductive biology literature.

Molecular formula: C63H83N15O12 | Molecular weight: 1302.45 g/mol | CAS: 374675-21-5 | Sequence: Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH2 | Amino acids: 10 | Purity: greater than or equal to 98% as verified by HPLC | Form: Lyophilised powder | Appearance: White to off-white lyophilised powder | Storage: -20°C desiccated

Loss-of-function mutations in KISS1 or GPR54 cause hypogonadotropic hypogonadism in humans, establishing kisspeptin signalling as the master gatekeeper of the HPG axis (Seminara et al., NEJM 2003; de Roux et al., PNAS 2003). GPR54 is a Gq/11-coupled GPCR activating phospholipase C, IP3-mediated calcium release, and PKC. KNDy neurons in the hypothalamic arcuate nucleus co-expressing kisspeptin, neurokinin B, and dynorphin generate pulsatile GnRH secretion — the signal driving pituitary LH and FSH release and downstream gonadal function.

Research applications include GPR54 binding kinetics and competitive displacement assays, calcium mobilisation functional assays in GPR54-expressing cells, GnRH pulse generator research in hypothalamic models, and HPG axis pharmacology studies examining gonadal steroid feedback regulation.

Supplied as a lyophilised powder. Reconstitute with bacteriostatic water. Store at -20°C. For laboratory and analytical research purposes only. Not for human or veterinary use.