FOXO4-DRI

FOXO4-DRI is a synthetic D-retro-inverso (DRI) peptide designed to interfere with the FOXO4-p53 protein-protein interaction that has been studied as a survival mechanism in senescent cells. Composed entirely of D-amino acids in the reverse sequence of the native FOXO4 helix-2 domain, it is fully resistant to proteolytic degradation while maintaining spatial mimicry of key side chain positions required for protein-protein interaction inhibition in the nucleus.

Molecular weight: approximately 2.8 kDa | Peptide type: D-retro-inverso (all D-amino acids, reversed sequence) | Target: FOXO4-p53 protein-protein interaction | Cell penetrating: Yes | Purity: greater than or equal to 98% as verified by HPLC | Form: Lyophilised powder | Appearance: White to off-white lyophilised powder | Storage: -20°C desiccated

Baar et al. (Cell, 2017) demonstrated that disrupting FOXO4-p53 interaction with a prototype DRI peptide restored apoptotic signalling in senescent cells in culture and mouse models, subsequently reducing senescence markers and SASP (senescence-associated secretory phenotype) factor secretion. The SASP includes IL-6, IL-8, TNF-alpha, and matrix metalloproteinases that drive tissue-level dysfunction in cellular senescence models.

FOXO4-DRI is used as a research tool for identifying senescent cell populations, studying apoptotic signalling in senescence models, characterising FOXO4-p53 interaction inhibition kinetics, and examining SASP modulation. Complementary research tools include NAD+ (sirtuin-mediated senescence pathways), MOTS-c (mitochondrial stress signalling relevant to ageing), and 5-Amino-1MQ (metabolic reprogramming in senescent cells).

Supplied as a lyophilised powder. Reconstitute in DMSO or sterile water. Store at -20°C. For laboratory and analytical research purposes only. Not for human or veterinary use.