TB-500

TB-500 is a synthetic analogue of Thymosin Beta-4 (Tb4), a naturally occurring 43 amino acid peptide encoded by the TMSB4X gene on the X chromosome. Thymosin Beta-4 is one of the most abundant intracellular peptides in mammalian cells and plays a central role in actin cytoskeletal dynamics through its LKKTET actin-binding motif at positions 17-22 of the sequence.

Molecular formula: C212H350N56O78S | Molecular weight: 4963.5 g/mol | CAS: 77591-33-4 | Gene: TMSB4X (X-linked) | Amino acids: 43 | Purity: greater than or equal to 98% as verified by HPLC | Form: Lyophilised powder | Appearance: White to off-white lyophilised powder | Storage: -20°C desiccated

The LKKTET hexapeptide motif binds monomeric G-actin with high affinity, sequestering it from the pool available for F-actin polymerisation and regulating the G/F-actin ratio in cell models. This mechanism directly influences lamellipodia formation and directional cell migration — processes studied extensively in wound healing scratch assays and transwell migration models. Published research has also examined TB-500 in cardiac epicardial progenitor cell mobilisation (Smart et al., Nature 2007), corneal epithelial cell repair, and neural progenitor biology.

Laboratory research applications include fibroblast and endothelial cell migration assays, angiogenesis tube formation models, cardiomyocyte survival and differentiation studies, corneal epithelial biology, and actin dynamics research comparing G/F-actin ratios under different treatment conditions. TB-500 is frequently studied alongside BPC-157 (NO and FAK-paxillin pathways) and MOTS-c (mitochondrial signalling) in tissue biology research programmes.

Supplied as a lyophilised powder. Reconstitute with bacteriostatic water. Soluble at physiological pH. Store at -20°C protected from light and moisture. For laboratory and analytical research purposes only. Not for human or veterinary use.